Research Projects
Some of the projects we’re currently working on:
Modulation of dopamine neuron activity by distinct inputs
Dopamine neurons receive inputs from dozens of brain regions, and many of these inputs co-release both neuropeptides and fast neurotransmitters. We use optogenetics, fiber photometry, gene mutagenesis, and more to uncover the organization, connectivity, and function of these inputs and dissect how fast transmitters and peptides work together to control dopamine neuron activity and regulate behavior.
Contributions of ion channels to dopamine neuron diversity and function
Distinct populations of dopamine neurons exhibit differential projections, physiology, and effects on behavior. One source of heterogeneity is the variable expression of ion channel genes that regulate neuronal excitability. Using CRISPR/Cas9 gene editing we can investigate the function of specific understudied ion channel genes and determine their role in establishing the physiology and responsivity of dopamine neurons.
Neural circuit regulation of consumption and energy balance
Hypothalamic and dopaminergic circuits converge to regulate many aspects of body weight and metabolism, including food consumption, locomotion, and reward signaling. We’re interested in understanding how these circuit elements interact with one another to control energy balance and consummatory behavior.
Experimental Approaches
Patch Clamp Electrophysiology
Fiber Photometry
Optogenetics
Multi-plex in situ
CRISPR gene editing
Viral Tracing
Behavior
Transcriptomics